ABSTRACT
Awareness on antioxidants and its significance in human healthcare has increased many folds in recent time. Increased demand requisite on welcoming newer and alternative resources for natural antioxidants. Seaweed associated pigmented bacteria screened for its antioxidant potentials reveals 55.5% of the organisms were able to synthesize antioxidant compounds. DPPH assay showed 20% of the organisms to reach a antioxidant zone of 1 cm and 8.3% of the strains more than 3 cm. Pseudomonas koreensis (JX915782) a Sargassum associated yellowish brown pigmented bacteria have better activity than known commercial antioxidant butylated hydroxytoluene (BHT) against DPPH scavenging. Serratia rubidaea (JX915783), an associate of Ulva sp. and Pseudomonas argentinensis (JX915781) an epiphyte of Chaetomorpha media, were also contributed significantly towards ABTS (7.2% ± 0.03 to 15.2 ± 0.09%; 1.8% ± 0.01 to 15.7 ± 0.22%) and FRAP (1.81 ± 0.01 to 9.35 ± 0.98; 7.97 ± 0.12 to 18.70 ± 1.84 μg/mL of AsA Eq.) respectively. 16S rRNA gene sequence analysis revealed bacteria that have higher antioxidant activity belongs to a bacterial class Gammaproteobacteria. Statistical analysis of phenolic contents in relation with other parameters like DPPH, ABTS, reducing power and FRAP are well correlated (p < 0.05). Results obtained from the current study inferred that the seaweed associated pigmented bacteria have enormous potential on antioxidant compounds and need to be extracted in a larger way for clinical applications.
Subject(s)
Antioxidants/metabolism , Aquatic Organisms/classification , Aquatic Organisms/metabolism , Gammaproteobacteria/classification , Gammaproteobacteria/metabolism , Seaweed/microbiology , Aquatic Organisms/genetics , Aquatic Organisms/isolation & purification , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Gammaproteobacteria/genetics , Gammaproteobacteria/isolation & purification , Molecular Sequence Data , Pigments, Biological/metabolism , /genetics , Sequence Analysis, DNAABSTRACT
During screening for oil-degrading bacteria in sediments of BahÝa Blanca estuary, a bacterial strain was isolated which uses a limited number of organic compounds, especially lineal hydrocarbons. The goals of this study were to characterize the strain and to evaluate its activity on crude oil. Based on phenotypic characteristics and the analysis of 16S rDNA sequences, it was determined that the strain is strongly related to Alcanivorax borkumensis. The strain was cultured, under quasicontinuous conditions, with crude oil as sole source of carbon and energy. After 30 days of incubation, n-alkane decrease reached 86. The isoalkanes pristane and phitane were degraded more slowly, but a decrease of about 63 was obtained. The strain studied would be useful for the bioremediation of the local coasts, so that this study will be continued in order to obtain its successful application in the environment.